Composite
SV40-4-2

Part:BBa_K2446034:Design

Designed by: Yijie Pan   Group: iGEM17_Fudan   (2017-10-21)


SV40_4_PIP promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is aseembled by the specfic DNA binding sites and Sv40 promoter through Biobrick assembly. We inset the DNA binding sites behind the Sv40 promoter. Then the DNA binding domain with KRAB can repress the expression of this promoter. The repression intensity can be adjusted by change the repeats of DNA binding sites.

Source

this part is assembled by Sv40 promoter BBa_K2446052 and 4*PIP DNA binding sites BBa_K2446011.

References

[1] M. Fussenegger et al., Streptogramin-based gene regulation systems for mammalian cells. Nature biotechnology 18, 1203--1208 (2000).

[2] R. Witzgall, E. O'Leary, A. Leaf, D. Onaldi, J. V. Bonventre, The Krüppel-associated box-A (KRAB-A) domain of zinc finger proteins mediates transcriptional repression. Proceedings of the National Academy of Sciences 91, 4514-4518 (1994).